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Today we’re diving into the world of expression systems with Iskandar Dib, of Validogen and we’ll explore the remarkable potential of Pichia pastoris, a methylotrophic yeast that’s revolutionizing pharmaceutical protein production and beyond.
From industrial enzymes to biomaterials, food, feed, and diagnostics, Pichia offers a reliable, scalable, and cost-efficient platform for recombinant protein expression.
Iskandar explains how biotechs can unlock Pichia’s full potential through Validogen’s sophisticated tools and strategies, fine-tuning protein expression with innovative genetic approaches and robust screening systems to deliver unmatched yields and quality.
01:06 Why Pichia is often overlooked or underestimated
03:29 The basics of Pichia pastoris
05:25 How Pichia compares to microbial hosts like E.coli
08:14 Key advantages over mammalian cell systems
10:21 Pichia as a system for secreted production
11:30 Pichia and post-translational modifications
14:30 The challenges of working with Pichia
16:25 Strain optimization and genetic engineering
17:29 Meet Iskandar Dib and Validogen
19:03 The UNLOCK PICHIA platform
24:00 Real-world applications of Pichia pastoris in biopharma
27:19 When Pichia outperforms expectations
28:25 Adapting Pichia for emerging fields
30:23 Advice for those considering Pichia pastoris for protein expression
33:23 Learn more about Validogen
This episode is sponsored by Validogen.
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To dive deeper into the topic:
Chapters
1. Pichia pastoris: the microbial protein expression platform you’re probably underestimating (00:00:00)
2. Why pichia is often overlooked or underestimated (00:01:06)
3. The basics of pichia pastoris (00:03:29)
4. How pichia compares to microbial hosts like E.coli (00:05:25)
5. Key advantages over mammalian cell systems (00:08:14)
6. Pichia as a system for secreted production (00:10:21)
7. Pichia and post-translational modifications (00:11:30)
8. The challenges of working with pichia (00:14:30)
9. Strain optimization and genetic engineering (00:16:25)
10. Meet Iskander Dib and Validogen (00:17:29)
11. The UNLOCK PICHIA platform (00:19:03)
12. Real-world applications of pichia pastoris in biopharma (00:24:00)
13. When pichia outperforms expectations (00:27:19)
14. Adapting pichia for emerging fields (00:28:25)
15. Advice for those considering pichia pastoris for protein expression (00:30:23)
16. Learn more about Validogen (00:33:23)
171 episodes
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