Saint Lucia is well on its way to strengthening its capacity in rapid HIV and syphilis testing and improving the quality of laboratory services on island, following the completion of the Master Trainers Training Programme which is part of the OECS Global Fund HIV/TB Project. The programme was implemented through the engagement of the Caribbean Med Labs Foundation, contracted to provide technical support and build sustainable laboratory training capacity. The Ministry of Health, Wellness and Elderly Affairs nominated four healthcare professionals to undergo certification as Master Trainers. These candidates participated in structured training sessions that combined both theoretical and practical components to ensure a high standard of competency and consistency in testing procedures. The Master Trainers will serve as the frontline point of contact, offering clarity, reassurance, and informed direction to those navigating the screening process.