))
Engineering the P450 Workhorse to Secure Supply Chains and Save Endangered Trees with Maria Astolfi
Manage episode 484208599 series 3643234
What if we could secure critical supply chains through bioengineering? What if the vaccines protecting millions worldwide didn't require harvesting 10,000 trees annually from Chilean mountains? Maria Astolfi is tackling this exact challenge through groundbreaking work with P450 enzymes.
Growing up surrounded by biodiversity shaped Maria's unique perspective on biotechnology. After co-founding the Amazon's first synthetic biology lab and working at Ginkgo Bioworks, she now conducts research in UC Berkeley's Jay Keasling laboratory. Her mission? Solving one of biomanufacturing's most persistent bottlenecks – engineering the notoriously difficult P450 enzymes that are crucial for producing complex natural products.
The stakes couldn't be higher. QS-21, a critical vaccine adjuvant, costs up to $200,000 per gram due to its complex extraction from Chilean trees. Beyond the environmental damage, this extractive approach creates volatile supply chains for essential medicines. Maria's innovative combination of machine learning and high-throughput robotics has already yielded a remarkable 3x improvement in enzyme activity – just the beginning of what's possible.
What makes Maria's vision truly transformative is how it reconnects biotechnology with biodiversity. By focusing first on this high-value target, she's creating infrastructure that could eventually transform production of countless natural products.
Listen to this episode for a glimpse into a future where advanced biotechnology and biodiversity protection go hand in hand.
00:00 Introduction to Vaccine Adjuvants and Climate Biotech
00:19 Welcome to the Climate Biotech Podcast
00:46 Meet Maria: From the Amazon to Biotech
01:50 Maria's Early Inspirations and Career Path
04:24 The Journey to Ginkgo Bioworks
10:49 Challenges and Innovations in Biomanufacturing
13:16 The Importance of Cytochrome P450 Enzymes
16:38 Scaling Sustainable Biomanufacturing
21:21 The Broader Impact of Biomanufacturing
25:25 Future Visions and Final Thoughts
33:33 Rapid Fire Questions and Closing Remarks
Chapters
1. Vaccine Supply Chain Crisis (00:00:00)
2. Maria's Background in the Amazon (00:02:45)
3. From Brazil to Ginkgo Bioworks (00:05:47)
4. The P450 Enzyme Challenge (00:10:48)
5. Machine Learning Meets Biological Engineering (00:17:42)
6. Sustainable Vaccine Production (00:21:40)
7. Future Vision for Biodiverse Supply Chains (00:28:28)
8. Final Thoughts and Advice (00:33:36)
20 episodes
Share
